Indonesia Journal of Biomedical Science is published by DiscoverSys Inc

Original Articles

The effect of hyaluronic acid on the changes in Matrix Metalloproteinase-1 (MMP-1), leukocytes level, and bacteriocide effects among patients with Wagner 3 and 4 diabetic foot ulcer

Ida Bagus Anangga Kharisma, I Nyoman Semadi, I Gede Raka Widiana
Online First: June 04, 2019

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Abstract

The effect of hyaluronic acid on the changes in Matrix Metalloproteinase-1 (MMP-1), leukocytes level, and bacteriocide effects among patients with Wagner 3 and 4 diabetic foot ulcer

Ida Bagus Anangga Kharisma, I Nyoman Semadi, I Gede Raka Widiana

Background: Diabetes Mellitus (DM) has become a major public health problem. A significant complication of Diabetes Mellitus is the emergence of diabetic foot ulcers that require special treatment in healing. Hyaluronic Acid plays a role in cell proliferation and cell movement and is needed in the process of wound healing. The main goal of treatment of diabetic ulcers in the foot is to achieve wound closure as quickly as possible, prevent recurrence and amputation.

Methods: A true experimental study with pre and post-test design has been conducted among 32 diabetic foot patients at Sanglah General Hospital which divided into 2 groups: Hyaluronic acid and NaCl 0.9% using consecutive techniques. The hyaluronic acid compared to NaCl 0.9% on MMP-1, leukocyte levels, and bacteriocide effects in patients with Wagner's diabetic foot 3 and 4 were assessed. Data were analyzed using SPSS version 20 software for Windows.

Results: The results showed that Hyaluronic Acid increased MMP-1 levels while NaCl 0.9% reduced MMP-1 levels significantly (P<0.05). Hyaluronic Acid was able to reduce leukocyte level significantly compared to NaCl 0.9% (P=0.006). A positive bacterial culture was also found markedly lesser in Hyaluronic Acid groups compared with NaCl 0.9% (P<0.001). Multivariate analysis using logistic and linear regression also showed significant differences in the MMP-1 and leukocyte level (P<0.001) and positive culture results using hyaluronic acids (OR: 0.020; 95% CI 0.003-0.166; P<0.001)

Conclusion: These results suggest that MMP-1 levels and leukocyte levels differed significantly between Hyaluronic Acid and NaCl 0.9% used. Hyaluronic Acid can increase MMP-1 levels in Wagner 3 and 4 diabetic care. The results of positive culture with Hyaluronic Acid used are less than NaCl 0.9%.

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Original Articles

Wound treatment with hyaluronic acid and silver sulfadiazine promote better epithelialization compared to polyurethane and normal saline in diabetic foot ulcer

Made Kurniawan Ardi Saputra, I Nyoman Semadi, I Gde Raka Widiana
Online First: August 01, 2019

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Abstract

Wound treatment with hyaluronic acid and silver sulfadiazine promote better epithelialization compared to polyurethane and normal saline in diabetic foot ulcer

Made Kurniawan Ardi Saputra, I Nyoman Semadi, I Gde Raka Widiana

Background: Diabetic foot ulcer, one of the chronic complication of diabetes mellitus that has a high morbidity and mortality rate. The primary conventional treatment for diabetic ulcer was debridement, followed by wound treatment as local control to promote healing and epithelization. There are many different methods on wound treatment; however only few types of research compared the efficacy. This study aims to compare the combination of hyaluronic acid and silver sulfadiazine with polyurethane or normal saline in diabetic foot ulcer treatment.

Method: A randomized clinical study was conducted to compare the efficacy of silver sulfadiazine with hyaluronic acid, polyurethane and normal saline in Wagner 3 and 4 diabetic foot ulcer as the main treatment until 3 weeks post debridement. Several variables assessed in this study, such as epithelialization area, granulation percentage, and ankle diameters. Appropriate statistical analysis was conducted with SPSS version 17 with p < 0.05 considered significant.

Result: The median tissue epithelialization area after 3 weeks of wound treatment on normal saline group was 1.62 cm² (IQR: 0.2 -2.22), on polyurethane group was 2.79 (IQR: 2.39 -3.19 cm²), and silver sulfadiazine with hyaluronic acid group was 3.1 (IQR: 2.1-4.1) cm² (p<0.05). There was no significant difference in the percentage of granulation (p=0.136) and ankle circumference (p=0.74) among treatment groups.

Conclusion: Silver sulfadiazine with hyaluronic acid promote the wound healing process in Wagner 3 and 4 diabetic foot ulcer after 3 weeks by promoting a better tissue epithelization compared to normal saline and polyurethane.

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Original Articles

Water Sport-Related Spine Injury in Bali: A Review and Preliminary Study

I Ketut Suyasa, Anak Agung Wiradewi Lestari, I Putu Yuda Prabawa, Ketut Kris Adi Marta
Online First: September 08, 2019

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Abstract

Water Sport-Related Spine Injury in Bali: A Review and Preliminary Study

I Ketut Suyasa, Anak Agung Wiradewi Lestari, I Putu Yuda Prabawa, Ketut Kris Adi Marta

Background: Spinal Injury is a devastating injury with life-long impact on an individual’s health and quality of life. Sporting activities, such as water sport, is also known contributed to the spinal injury. This study aims to evaluate the water sport-related spine injury (WS-RSI) which is occurred in Bali during 2017 as a preliminary study at BROS Hospital.

Methods: A cross-sectional study was conducted retrospectively among 55 patients who got an injury during water-sport activity in 2017 and admitted to BROS Hospital, Denpasar, Bali by medical records. There was 28 patient-related spine injury. Demographic status regarding age, sex, length of stays, hemoglobin (Hb), random blood glucose, nationality, country origin, treatment, patient’s status, history of alcohol intake, type of water sport, and the location of spine injury were recorded. Data were analyzed using SPSS version 25 for Windows in mean±SD, percentage, and odds ratio (OR) as well as statistically significant if P-value less than 0.05.

Results: Indonesian travelers were predominant in WS-RSI (53.6%), followed by China and Australia (17.9%), India, Japan, and European countries (3.6%, respectively). The average age of patients was higher in spine injury (47.89±15.98 years; P=0.047). Both sexes were equal (50%). There was no significant difference in Hemoglobin (Hb) (11.08±1.30 g/dL) and random blood glucose (100.65±21.37 mg/dL) (P>0.05) levels. However, there was a significant difference among nationality, type of treatment, patient’s status, and type of WS-RSI compared with non-spine injury (P<0.05). Based on variables, banana boat, foreign travelers, conservative treatment, and outpatient status were having a higher risk in WS-RSI (OR= 4.275; 5.143; 5.014; 7.389; P<0.05, respectively)

Conclusion: As a preliminary study, recent findings at BROS Hospital, Denpasar, Bali suggest that several factors are having a higher risk towards water sport-related spine injuries such as older age, banana boat, foreign travelers, conservative treatment, and outpatient status

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Case Reports

One stage release of circumferential constriction band in upper limb with multiple Z-plasty technique: a case report

I Gusti Putu Hendra Sanjaya, Agus Roy Rusli Hariantana Hamid, Gede Wara Samsarga
Online First: July 21, 2019

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Abstract

One stage release of circumferential constriction band in upper limb with multiple Z-plasty technique: a case report

I Gusti Putu Hendra Sanjaya, Agus Roy Rusli Hariantana Hamid, Gede Wara Samsarga

INTRODUCTION:

Congenital constriction band is an uncommon congenital abnormality with multiple disfiguring and disabling manifestations. The widely known management for the correction of congenital constriction band especially in circumferential constriction band is done in a multistage release of constriction band to eluding vascular and lymphatic embarrassment. The purpose of this case report was to present the outcome of one-stage release in terms of safety and aesthetic appearance.

CASE DESCRIPTION:

Women 28 year old came to outpatient plastic surgery clinic presented with circumferential constriction with no functional disability in her right arm since birth. Excision based on design of multiple Z-Plasty is started 2 cm proximal and distal from constricted band. The multiple Z- Plasty flaps are transposed around each other, changing the direction of the constriction band. Postoperatively, the extremity circulation was normal, and no edema was observed. The range of motion is within normal limits and aesthetically acceptable for the patient.

DISCUSSION: Patterson classified constriction ring syndrome into four types, and type II is the most common. In type II Paterson , there are normal full-layer skin structures and no important nerve or blood vessel was affected so an incision completely encircling an extremity would not interfere with distal circulation and provided strong evidence that one-stage resection of constriction band could be performed. Using multiple Z-Plasty for constriction band release leads to improvement of functional and cosmetic appearance of the defect.

CONCLUSION: A one-stage release of circumferential constriction band in the upper limb using multiple Z-Plasty is considered as a safe treatment with an aesthetically acceptable result for the patient

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