Original Articles

Cacao beans extract (Theobroma cacao L.) improve lipid profile but had no effect on blood nox concentration in dyslipidemia white male rat (Rattus norvegicus)

IA Dewi Wiryanthini , IWG Sutadarma, dr. Yuliana

IA Dewi Wiryanthini
Biochemestry Department Faculty of Medicine Udayana University Jln. PB Sudirman, Denpasar, Bali - Indonesia. Email: wiryanthini123@yahoo.com

IWG Sutadarma
Biochemestry Department Faculty of Medicine Udayana University Jln. PB Sudirman, Denpasar, Bali - Indonesia

dr. Yuliana
Anatomy Department Faculty of Medicine Udayana University Jln. PB Sudirman, Denpasar, Bali - Indonesia
Online First: July 02, 2017 | Cite this Article
Wiryanthini, I., Sutadarma, I., Yuliana, d. 2017. Cacao beans extract (Theobroma cacao L.) improve lipid profile but had no effect on blood nox concentration in dyslipidemia white male rat (Rattus norvegicus). Indonesia Journal of Biomedical Science 11(2): 1-5. DOI:10.15562/ijbs.v11i2.140


Background: Dyslipidemia is a disorder of lipoprotein metabolism, including overproduction of total cholesterol, Low-Density Lipoprotein (LDL), triglyceride and deficiency High-Density Lipoprotein (HDL), which cause decreased level of antioxidant enzyme and lead to oxidative stress, marked with increased of lipid peroxidation. Oxidative stress decreases blood nitrate and nitrite (NOx) which are the metabolites of nitric oxide (NO). Cacao beans extract rich in flavanols antioxidants such as catechin, epicatechin, and procyanidin. Objective: The aim of this experiment is to evaluate the effects of cacao beans extract (Theobroma cacao L) to blood profile lipid and NOx of dyslipidemia white rat (Rattus norvegicus). Methods: This experiment was a pretest-posttest control group design. Rats divided into four groups P0 control group only given high cholesterol food, P1 given 70 mg, P2 given 140 mg and P3 given 280 mg of cacao beans extract. Results: The administration of cacao beans extract significantly decreased (p=0,000) total cholesterol, triglyceride and LDL in group P1, P2 and P3 and significantly (p=0,000) increased HDL, although not significantly increased NOx in P1, P2, and P3 (p=0,486). Conclusion: Cacao beans could inhibit oxidative stress showed significantly decreased total cholesterol, triglyceride, LDL and significantly increased HDL but not able increased blood NOx concentration in dyslipidemia white rat.

References

Profil Kesehatan Indonesia 2005. Jakarta: Departemen Kesehatan Indonesia. 2007.

Mayes, P.A., and Botham, K.M. Lipids of Physiologic Significance. In: Murray, R.K., Granner, D.K., Mayes, P.A., and Rodwel, V.W (editors). Harper’s Illustrated Biochemistry. 26th. Ed. New York: McGraw Hill. 2003. p. 111-121.

Kaviarasan, K., Arjunan, M.M., and Pugalendi, K.V. Lipid Profile, Oxidant-antioxidant Status and Glycoprotein Components in Hyperlipidemic Patients With/without Diabetes. Clinica Chimica Acta. 2005.362:49-56

Vecera, R., et.al. Antioxidant Status, Lipoprotein Profile and Liver Lipids in Rats Fed on High-Cholesterol Diet Containing Currant Oil Rich in n-3 and n-6 Polyunsaturated Fatty Acids. Physiol. Res. 2003. 52:177-187.

Kusumawati, D. Bersahabat dengan Hewan Coba. Yogyakarta: Gadjah Mada University Press. 2004. p. 42 – 43

Nichols, J.L. The Laboratory Rat. Florida Atlantic University. 2003. [cited: 7 Januari 2010]. Available from: http://www.fau.edu/research/ovs/VetData/rat.php.

Sunarsih, E.S. and Prasetyastuti. Pengaruh Pemberian Juice Lidah Buaya terhadap Kadar Lipid Peroksida (MDA) Pada Tikus Jantan Hiperlipidemia. Majalah Obat Tradisional. 2008. 45(13):145-152.

Mortzzavimoghaddam, S.G., Zarban, A., and Rezvani, M. The Effect Of Vitamin E Supplementation On Lipid Profile And Oxidative Stress Markers In Hemodialysis Patients. ARYA Atherosclerosis Journal. 2007.3(3):139-142.

Jiyeon, K., Yeon, K.S., MinSook, L., and HongSeok, Ahn. Effect of vegetable juice supplementation on serum lipid profile and antioxidant activity in college women. Korean Journal of Community Nutrition. 2006.

McNulty.A. Differential Effects of Carotenoids on Lipid Peroxidation due to Membrane Interactions:X-rays Diffraction Analysis. Biochimica etBiophysica Acta. 2007. 1768:167-74.

Redón J, Oliva MR, Tormos C, Giner V, Chaves J, Iradi A, Sáez GT. Antioxidant Activities and Oxidative Stress By Products in Human Hypertension. Hypertension. 2003; 41: 1096-1101.

Granger JP, Alexander BT, Llinas MT, Bennet WA, Khalil RA. Pathophysiology of Hypertension During Preeclampsia Linking Placental Ischemia With Endothelial Dysfunction. Hypertension. 2001; 38(2): 718-722.

Cerielo A. Possible Role of Oxidative Stress in The Pathogenesis of Hypertension. Diabetes Care. 2008; 31(2): S181-S184.

R&D Systems. Nitric Oxide Synthases. 2000 [diakses 3 Juni 2011]; Diunduh dari : http://rndsystems.com/mini_review_detail_objectname_MR00_NOS.aspx

Lundberg JO, Weitzberg E. NO Generation From Nitrite and Its Role in Vascular Control. Arterioscler Thromb Vasc Biol. 2005; 25: 915-22.

Mao TK, Van De Water J, Keen CL, Schitz HH, Gershwin ME. Cocoa Flavanols and Procyanidins Promote Transforming Growth Factor-β1 Homeostasis in Peripheral Blood Mononuclear Cells. Exp Biol Med. 2003; 228: 93-9.

Niemenak, N., Rohsius, C., Elwers, S., Ndoumou, D.O., dan Lieberei, R. Original Article : Comparative Study of Different Cocoa (Theobroma cacao L.) Clones In Terms Of Their Phenolics and Anthocyanins Contents. Journal of Food Composition and Analysis. 2006. (19): 612-619.

Lecumberri, E., et al. Basic Nutritional Investigation : A Diet Rich In Dietary Fiber From Cocoa Improves Lipid Profile and Reduces Malondialdehyde in Hypercholesterolemic Rats. Nutrition. 2007. (23): 332-341.

Bender, D.A., and Mayes, P.A. Vitamins and Minerals. In: Murray, R.K., Granner, D.K., Mayes, P.A., and Rodwel, V.W (editors). Harper’s Illustrated Biochemistry. 26th. Ed. New York: McGraw Hill. 2003.p. 481-497.

Kwon SH. Anti-obesity and Hypolipidemik Effects of Black Soybean Anthocyanins. Journal of Medicinal Food. 2007; 10(3):552-6.

Sumardika IW, Jawi IM. Ekstrak Air Daun Ubijalar Ungu Memperbaiki Profil Lipid dan Meningkatkan Kadar SOD Darah Tikus yang Diberi Makanan Tinggi Kolesterol. Medicina. 2012;43:67-71.

Murphy, K.J.et al. Diet Containing Cocoa Powder With Flavanols and procyanidins Inhibits. Proceedings of The Nutrition Society of Australia. 2001. 25: S79.


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