Original Articles

Green tea extract resulted in a lower value of IL-6 and TNF-a expressions on rabbit model with Anterior Cruciate Ligament (ACL) rupture

Dendyningrat I G A G , IK Suyasa, Putu Astawa, KG Mulyadi Ridia

Dendyningrat I G A G
Department of Orthopaedic and Traumatology Udayana Faculty of Medicine/ Sanglah Hospital, Bali, Indonesia. Email: ndy_coolz87@yahoo.com

IK Suyasa
Department of Orthopaedic and Traumatology Udayana Faculty of Medicine/ Sanglah Hospital, Bali, Indonesia

Putu Astawa
Department of Orthopaedic and Traumatology Udayana Faculty of Medicine/ Sanglah Hospital, Bali, Indonesia

KG Mulyadi Ridia
Department of Orthopaedic and Traumatology Udayana Faculty of Medicine/ Sanglah Hospital, Bali, Indonesia
Online First: June 13, 2018 | Cite this Article
I G A G, D., Suyasa, I., Astawa, P., Ridia, K. 2018. Green tea extract resulted in a lower value of IL-6 and TNF-a expressions on rabbit model with Anterior Cruciate Ligament (ACL) rupture. Indonesia Journal of Biomedical Science 12(2). DOI:10.15562/ijbs.v12i2.159


Background: Anterior Cruciate Ligament (ACL) rupture often occur at a young age. There is still debate about the timing of the surgical interventions. Many studies recommended that ACL reconstruction should be done after the third week of injury. Provision of green tea extract on ACL rupture is expected to more rapidly reduce the inflammatory reaction, which is characterized by lower IL-6 levels and lower TNF-α expression at the first, second and third week as compared with no administration of green tea extract so that surgical intervention can be done earlier. Aim: To evaluate the effect of green tea extract in IL-6 expression and TNF-α level. Methods: This research is an experimental randomized post-test only group design with 24 ACL-transected rabbit samples and divided into six groups. Three treatment groups and three control groups were further divided into first, second, and third week groups. The treatment group was administered with 300mg/kgBW green tea extract and control group was not administered. IL-6 and TNF-α were examined in each treatment and control group during the first, second and third weeks. Results: Oneway-ANOVA study showed that serum IL-6 in the treatment group was lower as compared to control group at first, second, and the third week with p = 0.000 (p <0.05). Kruskal-Wallis study for TNF-α expression in the treatment group was lower than the control group at first, second, and the third week with p = 0.000 (p <0.05). Conclusion: Green tea extract resulted in lower IL-6 expression and lower TNF-α level than with those not administered green tea extract.

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