Original Articles

The difference of nitric oxide (NO) and serum creatinine (SC) levels in hemodialyzed and non-hemodialyzed chronic kidney disease patients at H. Adam Malik Hospital, Medan, Indonesia

Ruslan Dame Hotmauli Marpaung , Ratna Akbari Ganie, Alwi Thamrin Nasution

Ruslan Dame Hotmauli Marpaung
Department of Clinical Pathology , Faculty of Medicine, University of North Sumatra, H.Adam Malik Hospital Medan,Indonesia.. Email: m_dame73@yahoo.com

Ratna Akbari Ganie
Department of Clinical Pathology , Faculty of Medicine, University of North Sumatra, H.Adam Malik Hospital Medan,Indonesia.

Alwi Thamrin Nasution
Department of Internal Medicine, Faculty of, Medicine University of North Sumatra, Medan, H.Adam Malik Hospital Medan, Indonesia.
Online First: June 01, 2019 | Cite this Article
Marpaung, R., Ganie, R., Nasution, A. 2019. The difference of nitric oxide (NO) and serum creatinine (SC) levels in hemodialyzed and non-hemodialyzed chronic kidney disease patients at H. Adam Malik Hospital, Medan, Indonesia. Indonesia Journal of Biomedical Science 13(1): 22-25. DOI:10.15562/ijbs.v13i1.173


Background: Chronic Kidney Disease is structural or functional abnormalities of the kidney that last more than 3 months, with or without decreased glomerular filtration rate (GFR) which can be exacerbated by reduced production of Nitric Oxide (NO) or increased serum creatinine level. Hemodialysis is a removal process of solute from the circulation of patients with an impaired renal function to the fluid dialysate by diffusion process through the semi-permeable membrane. This study aims to determine the difference of the Nitric Oxide (NO) and serum creatinine (SC) levels between regular hemodialysis and non-hemodialysis CKD patients.

Methods: A cross-sectional study was conducted at Outpatient and Inpatient Section of Nephrology Division, Internal Medicine Department, H. Adam Malik Hospital, Medan during July-August 2017 among 27 respondents who undergone regular Hemodialisa (HD) and 27 respondents in non-Hemodialisa (non-HD) group. The patients that fulfill the inclusion criteria of the study were enrolled. Nitric Oxide examination was carried out by using Chemwell analyzer with a double-antibody sandwich method using Enzyme method -Linked Immunosorbent Assay (ELISA).

Results: There was no significant difference of NO-levels between HD groups (1476.14± 1431.1 µM) with non HD (1123.06 ± 1150.83 µM) (P= 0.250). However, there was a significant difference of creatinine level between HD groups (11.8±3.26 pg/mL) with a non-HD group (5.71±5.03 pg/mL) (P = 0.000).

Conclusion: No significant difference of NO levels were found in HD patients and non-HD CKD patients. However, a current study exhibits a significant relationship between SC levels of CKD patients between both groups.

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