Original Articles

Amplification of 0.7 Kb Fragment katG Gene from Clinical Multi-Drug Resistant Tuberculosis (MDR-TB) Isolate in Bali

A. W. Dwiputri , K. Ratnayani, S. C. Yowani

A. W. Dwiputri
Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana University. Email: awdwiputri@gmail.com

K. Ratnayani
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University

S. C. Yowani
Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Udayana University
Online First: July 26, 2015 | Cite this Article
Dwiputri, A., Ratnayani, K., Yowani, S. 2015. Amplification of 0.7 Kb Fragment katG Gene from Clinical Multi-Drug Resistant Tuberculosis (MDR-TB) Isolate in Bali. Indonesia Journal of Biomedical Science 9(2). DOI:10.15562/ijbs.v9i2.21


The last decade has seen a particular increase in the occurrence of drug-resistant (DR-TB) and multi-DR strains, such as Isoniazid (INH) resistant strains of M. tuberculosis.  INH resistance is more frequently associated with mutations in the katG gene. Detection of katG gene mutations can be performed by PCR technique, followed by sequences. The aim of this study is to amplify katG gene region (0,7 Kb) from clinical isolate of MDR-TB in Bali. DNA isolation for PCR was done by Boom method and katG gene amplification was performed under the following conditions: predenaturation at 95oC for 15 min; fourty cycles of denaturation at 94oC for 1 min, annealing at 56oC for 1 min, extension at 72oC for 2 min; final extension at 72oC for 10 min. The amplicons were detected by 1,5% agarose gel electrophoresis and showed a specific band size at 0.7 kb. This suggests that the fragment of katG gene has been successfully amplified in these area.

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