Original Articles

In vivo antioxidant and hepatoprotective activity of ethanol leaf extract of Senna hirsuta Mill

Fred Coolborn Akharaiyi , Lucky Efe Isunu

Fred Coolborn Akharaiyi
Microbiology Department, Edo University Iyamho, KM 7, Auchi-Abuja Road, Iyamho-Uzairue, Edo State, Nigeria. Email: akharaiyi.fred@edouniversity.edu.ng

Lucky Efe Isunu
Author affiliations; Biotechnology Department, Federal University of Technology, Akure, Ondo State, Nigeria
Online First: September 29, 2020 | Cite this Article
Akharaiyi, F., Isunu, L. 2020. In vivo antioxidant and hepatoprotective activity of ethanol leaf extract of Senna hirsuta Mill. Indonesia Journal of Biomedical Science 14(2): 55-61. DOI:10.15562/ijbs.v14i2.233


Purpose: Liver diseases as a problem all over the world has led to many deaths because the conventional drugs known for the cure are inadequate to effect perfect healing. This study aims to evaluate the in vivo antioxidant and hepatoprotective potentials of ethanol leaf extract of Senna hirsuta using mice model.

Methods: After the initial effect of hepatic injury in mice with 1 g/kg body weight of paracetamol in a single dose for three days, 200, 400, 800, 1000 and 2000 mg/kg body weight of extract concentrations were used for treating the mice for five days. Blood and liver tissue samples were collected for analyses

Results: The results obtained proved that paracetamol elicited toxicity in the mice. The red blood cells mean count in the negative control (7.67±0.92 million/mm3) was higher than the values obtained from the positive control (4.36±0.12 million/ mm3). The WBC mean values of 3.50±0.18 thousand/mm3 from negative control were lower than the positive control group (9.62±0.39 thousand/ mm3). Also, a higher value above the permissible level (11-19 %) was obtained in the haemoglobin estimation of the positive control (20.11%) than the negative control (11.50%). Values of the positive control and extract treated were higher than the negative control value while lower values were recorded in GSH and CAT in positive control and extract treated. Higher biochemical values were recorded in the positive control, and the extract administered except for albumin and protein. However, the injuries caused that manifested in the mice haematology, biochemical and liver disorders were significantly improved with the 200 – 2000 mg of extract concentrations.

Conclusion: Leaf extract of Senna hirsuta proved the hepatoprotective activity of the employed extracts. The observation did by biochemical, hematological indices, and recovery improvement in liver function by histologic findings. Therefore, established that S. hirsuta ethanol leaf extract has hepatoprotective affinity over paracetamol-induced liver toxicity.

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