Original Articles

Effication of amniotic membrane topical stem cell-conditioned medium combined with YAG erbium fractional laser 2940 nm in photoaging skin

I Gusti Ayu Agung Praharsini, I Gusti Ayu Agung Elis Indira, Cita Rosita Sigit Prakoeswa , Made Swastika Adiguna, Luh Mas Rusyati, Menul Ayu Umborowati, Irmadita Citrashanty, Maylita Sari

I Gusti Ayu Agung Praharsini
Dermatology and Venereology Department, Faculty of Medicine, Universitas Udayana-Sanglah General Hospital, Bali-Indonesia

I Gusti Ayu Agung Elis Indira
Dermatology and Venereology Department, Faculty of Medicine, Universitas Udayana-Sanglah General Hospital, Bali-Indonesia

Cita Rosita Sigit Prakoeswa
Dermatology and Venereology Department, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo Hospital, Surabaya, Indonesia. Email: drcita.rosita@gmail.com

Made Swastika Adiguna
Dermatology and Venereology Department, Faculty of Medicine, Universitas Udayana-Sanglah General Hospital, Bali-Indonesia

Luh Mas Rusyati
Dermatology and Venereology Department, Faculty of Medicine, Universitas Udayana-Sanglah General Hospital, Bali-Indonesia

Menul Ayu Umborowati
Dermatology and Venereology Department, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo Hospital, Surabaya, Indonesia

Irmadita Citrashanty
Dermatology and Venereology Department, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo Hospital, Surabaya, Indonesia

Maylita Sari
Dermatology and Venereology Department, Faculty of Medicine, Universitas Airlangga-Dr. Soetomo Hospital, Surabaya, Indonesia
Online First: July 26, 2020 | Cite this Article
Praharsini, I., Indira, I., Prakoeswa, C., Adiguna, M., Rusyati, L., Umborowati, M., Citrashanty, I., Sari, M. 2020. Effication of amniotic membrane topical stem cell-conditioned medium combined with YAG erbium fractional laser 2940 nm in photoaging skin. Indonesia Journal of Biomedical Science 14(2): 44-47. DOI:10.15562/ijbs.v14i2.243


Background: Skin ageing is a natural process in humans that is influenced by endogenous and exogenous factors. Nowadays, many people have the concept of health and skin beauty considered as one of the main factors that represent the perception of health in human wellness. Therefore, various anti-ageing therapy strategies that have been developed over the past few years include stem cell therapy. Amniotic Membrane Stem Cells (AMSC) are stem cell products taken from amniotic membranes that contain various proteins and have low immunogenicity. Objective: Proving that Amniotic Membrane Stem Cell (AMSC) metabolite products can improve the clinical picture of photoaging.

Method: Study design using one group pre and post-test design model. Samples were obtained by consecutive sampling and the number of samples was 33 people. Clinical examination of patients with the Glogou scale followed by an one smart skin analyzer. Application of AMSC products 3 times daily at intervals of 2 weeks after the Erbium YAG Fractional 2940 nm laser and evaluation were carried out at weeks 0, 4, and 8.

Results: Pore diameter showed significant improvement (p<0.001) from first to fifth visit (6.52 ± 0.57 vs 5.79 ± 0.7), wrinkles showed significant improvement (p<0.001) from baseline copared to fifth visit (4.28 ± 0.38 vs 4.09 ± 0.20), pigment, moisture, and skin elasticity showed significant improvement (p<0.001) from the (baseline) compared to fifth consecutive visits (4.35 ± 1.41 vs 3.09 ± 0.68), (42.2 ± 7.64 vs 59.1 ± 7.94), (48.2 ± 14.1 vs 61.7 ± 13.1) respectively. Sebum T Zone and U zone showed significant improvement (p<0.001) from (baseline) and compared to fifth consecutive visit (12325 ± 10616.8 vs 2047.5 ± 1887.9) and (8529.8 ± 9026.8 vs 1560.6 ± 895.7). Side effects of urticaria were found in 1 person.

Conclusion: AMSC product therapy can be used as a potential for anti-ageing therapy.

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