Original Articles

Ozonated aloe vera oil improve wound healing by decreasing neutrophil count and wound diameter in Sprague dawley rats with full-thickness skin defect

Reza Vahlepi, Muhamad Thohar Arifin , Muhammad Nur, Muflihatul Muniroh, Neni Susilaningsih

Reza Vahlepi
Biomedical Magister Program, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Muhamad Thohar Arifin
Biomedical Magister Program, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia Neurosurgery Department, Faculty of Medicine, Universitas Diponegoro-Kariada General Hospital, Semarang, Indonesia. Email: thohar@fkundip.ac.id

Muhammad Nur
Physic Division, Faculty of Science and Mathematics, Universitas Diponegoro, Semarang, Indonesia

Muflihatul Muniroh
Biomedical Magister Program, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia Anatomy and Histology Department, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Neni Susilaningsih
Biomedical Magister Program, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia Physiology Department, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia
Online First: September 28, 2020 | Cite this Article
Vahlepi, R., Arifin, M., Nur, M., Muniroh, M., Susilaningsih, N. 2020. Ozonated aloe vera oil improve wound healing by decreasing neutrophil count and wound diameter in Sprague dawley rats with full-thickness skin defect. Indonesia Journal of Biomedical Science 14(2): 48-54. DOI:10.15562/ijbs.v14i2.253


Introduction: Wounds fatality is possible on instances where treatment is not conducted correctly. Moreover, Aloe Vera possesses anti-microbial activities, while ozone has bactericidal and antioxidant effects. This study is, therefore, aimed at assessing the number of neutrophils and the diameter of full-thickness wounds, following the multilevel dose administration of ozonated aloe vera oil.

Methods: This is an experimental study, based on a post-test only control group design, with treatment and control groups. A total of 50 Sprague Dawley rats (250 ± 50 grams) were randomly divided into 10 units, the control was administered aloe vera oil, gentamicin ointment, and the treatment with ozonated aloe vera oil at varied dose of 600, 1200, and 1800 mg/ml. Subsequently, five groups were terminated on the 3rd day, while the remaining groups were euthanized on the 7th day. The neutrophil counts were obtained after histological examination with Hematoxylin Eosin (HE) stain, while the wound diameter was measured using calipers.

Results: There were significant differences between the control and treatment groups. This was evidenced in the decreased neutrophil count after administering ozonated aloe vera oil at a dose of 1800 mg/ml, compared to 1200 and 600 mg/ml, and also aloe vera oil and gentamycin ointment. Furthermore, wound diameter was substantially reduced with all ozonated treatments, than the aloe vera oil and gentamycin treatments.

Conclusion: Ozonated aloe vera oil is characterized by the capacity to improve wound healing

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