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Effect of high-fat high fructose diet and carbon tetrachloride on high sensitivity C reactive protein (hsCRP) levels male Wistar rat

  • Delvina Tandiari ,
  • Ika Yustisia ,
  • Arif Santoso ,
  • Husni Cangara ,
  • Firdaus Hamid ,
  • Nu’man AS Daud ,


Background: The consumption of a high-fat diet affects fat metabolism in the body, and it gets progressively worse by adding high fructose to the diet. Similarly, the chemical compound carbon tetrachloride (CCl4) can cause toxic effects in multiple organs. High sensitivity C reactive protein (hsCRP) is a micro-inflammatory marker used as a primary preventive tool against several diseases. This study aimed to determine the impact of giving a high-fat, high-fructose diet (HFHFD) and CCl4 on hsCRP levels of Wistar rats.

Methods: An experimental study among 24 male Wistar rats aged 8-12 weeks divided into four groups. Rats were fed an HFHFD and were either administered CCl4 biweekly (0.08 mL/kg, peritoneal) or not. The other two groups were fed with standard chow meal with CCl4 injection, and the un-injected group stands as a control group. All groups were monitored for eight weeks, and serum parameters were examined. Data were analyzed using SPSS version 17 for Windows. P-value <0.05 was significant.

Results: The intake of a high fat and high-fructose diet for eight weeks was proven to have higher levels of hsCRP than those without the diet. Similar to the administration of 0.8 ml/kg peritoneal CCl4 biweekly shown to have higher levels of hsCRP compared to the control group. This study also showed increased body weight except for the group that received a standard meal and CCl4 peritoneal injection.

Conclusion: High-fat high fructose diet and carbon tetrachloride had a significant effect on hsCRP level.



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How to Cite

Tandiari, D., Yustisia, I., Santoso, A., Cangara, H., Hamid, F., & Daud, N. A. (2021). Effect of high-fat high fructose diet and carbon tetrachloride on high sensitivity C reactive protein (hsCRP) levels male Wistar rat. Indonesia Journal of Biomedical Science, 15(1), 71–74.