Skip to main content Skip to main navigation menu Skip to site footer

Anti-hyperurisemic Activity of Combination of Beetroot Powder (Beta vulgaris L.) And Allopurinol in Potassium Oxonate-Induced White Rats

Abstract

Introduction: Hyperuricemia is an abnormally high quantity of uric acid in the blood. Allopurinol is a type of xanthine oxidase inhibitor commonly used to lower uric acid levels in the general population. However, large doses of allopurinol can produce harmful effects, one of which being hepatotoxicity. The antioxidant activity of beetroot powder is high, and it has an antihyperuricemic effect. Giving beetroot powder and allopurinol together is intended to minimize allopurinol's adverse effects and give a positive increase in lowering uric acid levels. This study aims to evaluate the anti hyperuricemia effect of beetroot powder (Beta vulgaris L.) and allopurinol combination in white rats (Rattus novergicus strain Sprague Dawley) induced by potassium oxonate.

Methods: This an experimental study with a randomized pre and post-test control group design. In this experiment, twenty rats were placed into four treatment groups; KP = Positive Control (hyperuricemic rats) + standard feed and drink; G1 = hyperuricemic rats + allopurinol 1,8 mg/Kg BW/day); G2 = Hyperuricemic rats + beet powder 1.56 g/Kg BW/day; G3 = hyperuricemic rats + allopurinol 1,8 mg/Kg BW/day and beetroot powder 1,56 g/Kg BW/day. On day 0 and day 28, the amounts of uric acid were measured. Data obtained were analyzed using SPSS version 23 for windows.

Results: The mean pre-post changes in uric acid levels in each group were KP = 8.97 ± 0.48 mg/dl, G1 = 2.06 ± 0.15 mg/dl, G2 = 2.24 ± 0.10 mg/dl, and G3 = 2.11 ± 0.86 mg/dl. The mean pre-post changes in MDA levels in each group were KP = 8.14 ± 0.39 nmol/mL, G1 = 2.01 ± 0.38 nmol/mL, G2 = 3.13 ± 0.30 nmol/mL, and G3 = 2.35 ± 0.19 nmol/mL.

Conclusion: Beetroot powder and allopurinol combination given for 28 days significantly reduced uric acid and MDA levels in potassium oxonate-induced white rats.

Section

References

  1. Mehmood A, Zhao L, Wang C, Nadeem M, Raza A, Ali N, et al. management of hyperuricemia through dietary polyphenols as a natural medicament: A comprehensive review. Crit Rev Food Sci Nutr. 2019;59(9):1433–55.
  2. Riskesdas. Laporan Nasional Riskesdas 2018. Badan Penelitian dan Pengembangan Kesehatan. Badan Penelitian dan Pengembangan Kesehatan Kementerian RI tahun 2018.; 2018. p. 198.
  3. You L, Liu A, Wuyun G, Wu H, Wang P. Prevalence of Hyperuricemia and The Relationship Between Serum Uric Acid and Metabolic Syndrome in the Asian Mongolian Area. J Atheroscler Thromb. 2014;21(4):355–65.
  4. Jin M, Yang F, Yang I, Yin Y, Luo JJ, Wang H, et al. Uric Acid, Hyperuricemia and Vascular Diseases. Front Biosci (Landmark Ed. 2012;17:656–69.
  5. Newsholme P, Cruzat VF, Keane KN, Carlessi R, de Bittencourt PIH. Molecular Mechanisms of ROS Production and Oxidative Stress in Diabetes. Biochem J. 2016;473(24):4527–50.
  6. Haidari F, Keshavarz SA, Shahi MM, Mahboob SA, Rashidi MR. Effects Of Parsley (Petroselinum Crispum) And Its Flavonol Constituents, Kaempferol And Quercetin, On Serum Uric Acid Levels, Biomarkers Of Oxidative Stress and Liver Xanthine Oxidoreductase Aactivity Inoxonate-Induced Hyperuricemic Rats. Iran J Pharm Res. 2011;10(4):811–9.
  7. Sarvaiya VN, Sadariya KA, Pancha PG, Thaker AM, Patel AC. Evaluation of Antigout Activity of Phyllanthus Emblica Fruit Extracts on Potassium Oxonate-Induced Gout Rat Model. Vet World. 2015;8:1230–6.
  8. Li L, Teng M, Liu Y, Qu Y, Zhang Y, Lin F, et al. Anti-Gouty Arthritis and Antihyperuricemia Effects of Sunflower (Helianthus annuus) Head Extract in Gouty and Hyperuricemia Animal Models. Biomed Res Int. 2017;2017:1–9.
  9. Zhou Y, Zhao M, Pu Z, Xu G, Li X. Relationship between oxidative stress and inflammation in hyperuricemia. Medicine (Baltimore). 2018;97(49):e13108.
  10. Su Q, Su H, Nong Z, Li D, Wang L, Chu S, et al. Hypouricemic and Nephroprotective Effects of an Active Fraction from Polyrhachis Vicina Roger on Potassium Oxonate-Induced Hyperuricemia in Rats. Kidney Blood Press Res. 2018;43(1):220–33.
  11. Alqahtani MJ, Alshamrani AM. Overview of Allopurinol Decisions in Primary Care: A Narrative Review. Egypt J Hosp Med. 2018;72(2):3909–13.
  12. Juraschek SP, Miller ER, Gelber AC. Effect of oral vitamin C supplementation on serum uric acid: A meta-analysis of randomized controlled trials. Arthritis Care Res. 2012;63(9):1295–306.
  13. Anggraini DI, Suprijono A, Wahyusetyaningrum SL. Mineral Dalam Buah Naga (Hylocereus Undatus (Haw.) Britt. & Rose) Sebagai Penurun Asam Urat. J Ilm Kesehat. 2013;5(1).
  14. Suhendi A, Nurcahyanti, Muhtadi, Sutrisna EM. Aktivitas antihiperurisemia ekstrak air jinten hitam ( Coleus ambonicus Lour ) pada mencit jantan galur balb-c dan standardisasinya Antihyperurisemia activity of water extract of black seed ( Coleus ambonicus Lour ) in balb-c mice and its standardi-. Maj Farm Indones. 2011;22(2):77–84.
  15. Aprillinda FW, Paramasari D, Brian W. The Effect of Red Guava Juice (Psidium guajava Linn.) in Decreasing Uric Acid and Creatinine Levels of White Mice (Mus musculus) with Hyperuricemia. Bali Med J. 2018;7(2):323–9.
  16. Wells BG., DiPiro JT, Schwinghammer TL, DiPiro C V. Pharmacotherapy Handbook. New York: McGraw-Hill-Open Acces Journal; 2012.
  17. Luo C, Lian X, Hong L, Zou J, Li Z, Zhu Y, et al. High Uric Acid Activates the ROS-AMPK Pathway, Impairs CD68 Expression and Inhibits OxLDL-Induced Foam-Cell Formation in a Human Monocytic Cell Line, THP-1. Cell Physiol Biochem. 2016;40(3–4):538–48.
  18. Maiuolo J, Oppedisano F, Gratteri S, Muscoli C, Mollace V. Regulation of uric acid metabolism and excretion. Int J Cardiol. 2016;213:8–14.
  19. Kensara OA. Protective effect of vitamin C supplementation on oxonate-induced hyperuricemia and renal injury in rats. Int J Nutr Metab. 2013;5(June):61–6.
  20. Wong YM, Siow LF. Effects of Heat, Ph, Antioxidant, Agitation and light On Betacyanin Stability Using Red-Fleshed Dragon Fruit (Hylocereus polyrhizus) Juice and Concentrate as Models. J Food Sci Technol. 2015;52(5):3086–92.
  21. Novatama SM, Kusumo E, Supartono. Identifikasi Betasianin dan Uji Antioksidan Ekstrak Buah Bit Merah. Indo J Chem Sci. 2016;5(3):217–20.
  22. Asra R, Rusdi R, Nofrianti R. Physicochemical Study of Mangosteen (Garcinia mangostana L.) peel Extract as Coloring Agent in Tablet Formulation. J Pharm Sci. 2020;3(1):22–32.
  23. Al-azzawie HF, Abd SA. Effects of Crude Flavonoids from Ginger ( Zingiber officinale), on Serum Uric Acid Levels, Biomarkers of Oxidative Stress and Xanthine Oxidase Activity in Oxonate-Induced Hyperuricemic Rats. Int J Adv Res. 2015;2(6):55–61.
  24. Wruss J, Waldenberger G, Huemer S, Uygun P, Lanzerstorfer P, Müller U, et al. Compositional Characteristics of Commercial Beetroot Products and Beetroot Juice Prepared from Seven Beetroot Varieties Grown in Upper Austria. J Food Compos Anal. 2015;42(3):46–55.

How to Cite

Wulandari, A., Dirgahayu, P., & Wiboworini, B. (2021). Anti-hyperurisemic Activity of Combination of Beetroot Powder (Beta vulgaris L.) And Allopurinol in Potassium Oxonate-Induced White Rats. Indonesia Journal of Biomedical Science, 15(2), 164–168. https://doi.org/10.15562/ijbs.v15i2.319

HTML
32

Total
1

Share

Search Panel

Anggraini Wulandari
Google Scholar
Pubmed
IJBS Journal


Paramasari Dirgahayu
Google Scholar
Pubmed
IJBS Journal


Budiyanti Wiboworini
Google Scholar
Pubmed
IJBS Journal