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Analysis of Interleukin-6 level in Serum and Histopathology Changes of Cardiovascular Tissue on Male Wistar Rat with Nonalcoholic Fatty Liver Disease (NAFLD)

  • Mylda Pratiwi Ahmadwirawan ,
  • Husni Cangara ,
  • Arif Santoso ,
  • Marhaen Hardjo ,
  • Endy Adnan ,
  • Firdaus Hamid ,


Background: Nonalcoholic fatty liver disease (NAFLD) is the most common chronic hepatopathy and a global health issue. Interleukin-6 (IL-6) in liver pathology is very complex, and its participation in the development of NAFLD remains unclear. This study aimed to analyze the relationship between NAFLD and IL-6 as one of inflammaging biomarkers along with CVD histopathology.

Methods: This study used a post-test control group design. The subject of this study was 14 male Wistar rats and divided into two groups. The control group was given standard diet and the NAFLD-modelling group was given 8 weeks high-fat-high-fructose diet. Both groups will be terminated at the 8th week for organ histopathological examination.

Results: The IL-6 levels in NAFLD rats (16.14±6.46ng/ml) and non-NAFLD rats (11.28±3.49ng/ml). The blood vessel wall thickness of NAFLD (1.47±0.33 µm) and non-NAFLD rats (1.70±0.28 µm). The diameter of the blood vessels of NAFLD rats (14.06±3.30 m) and non-NAFLD rats (15.66±1.02 m). It was found that there were differences in cardiac histopathology (hemorrhagic (p:0.003), degenerated (p:0.036), and hyperemic(p:0.003)) between NAFLD and non-NAFLD modeling rats. As for IL-6 levels, blood vessel wall thickness, diameter, and heart histopathology (necrosis) there was no difference between NAFLD and non-NAFLD modeling rats (p>0.05).

Conclusion: It has shown that NAFLD rats significantly caused cardiac histopathological changes. The levels of IL-6 tend to be higher in NAFLD rats but not significant, as well as the diameter of coronary artery shows a small tendency in NAFLD rats.



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How to Cite

Ahmadwirawan, M. P., Cangara, H., Santoso, A., Hardjo, M., Adnan, E., & Hamid, F. (2021). Analysis of Interleukin-6 level in Serum and Histopathology Changes of Cardiovascular Tissue on Male Wistar Rat with Nonalcoholic Fatty Liver Disease (NAFLD). Indonesia Journal of Biomedical Science, 15(2), 174–179.