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Analysis of Interleukin-6 level in Serum and Histopathology Changes of Cardiovascular Tissue on Male Wistar Rat with Nonalcoholic Fatty Liver Disease (NAFLD)

  • Mylda Pratiwi Ahmadwirawan ,
  • Husni Cangara ,
  • Arif Santoso ,
  • Marhaen Hardjo ,
  • Endy Adnan ,
  • Firdaus Hamid ,


Abstract

Background: Nonalcoholic fatty liver disease (NAFLD) is the most common chronic hepatopathy and a global health issue. Interleukin-6 (IL-6) in liver pathology is very complex, and its participation in the development of NAFLD remains unclear. This study aimed to analyze the relationship between NAFLD and IL-6 as one of inflammaging biomarkers along with CVD histopathology.

Methods: This study used a post-test control group design. The subject of this study was 14 male Wistar rats and divided into two groups. The control group was given standard diet and the NAFLD-modelling group was given 8 weeks high-fat-high-fructose diet. Both groups will be terminated at the 8th week for organ histopathological examination.

Results: The IL-6 levels in NAFLD rats (16.14±6.46ng/ml) and non-NAFLD rats (11.28±3.49ng/ml). The blood vessel wall thickness of NAFLD (1.47±0.33 µm) and non-NAFLD rats (1.70±0.28 µm). The diameter of the blood vessels of NAFLD rats (14.06±3.30 m) and non-NAFLD rats (15.66±1.02 m). It was found that there were differences in cardiac histopathology (hemorrhagic (p:0.003), degenerated (p:0.036), and hyperemic(p:0.003)) between NAFLD and non-NAFLD modeling rats. As for IL-6 levels, blood vessel wall thickness, diameter, and heart histopathology (necrosis) there was no difference between NAFLD and non-NAFLD modeling rats (p>0.05).

Conclusion: It has shown that NAFLD rats significantly caused cardiac histopathological changes. The levels of IL-6 tend to be higher in NAFLD rats but not significant, as well as the diameter of coronary artery shows a small tendency in NAFLD rats.

Keywords: Interleukin-6 (IL-6) Cardiovascular Histopathology Non-alcoholic Fatty Liver Disease (NAFLD) Wistar
Section

References

  1. Giandomenico B, Ricci F, Mantini C, et al. Nonalcoholic fatty liver disease and cardiovascular disease phenotypes. Sage Open Medicine. 2020;8:1-15.
  2. Cai J, Zhang XJ, Ji YX, et al. Nonalcoholic Fatty Liver Disease Pandemic Fuels the Upsurge in Cardiovascular Diseases. Circulation Research. 2020;126:679-704.
  3. Kasper P, Martin A, Lang S, et al. NAFLD and cardiovascular diseases: a clinical review. Clin Res Cardiol. 2020; 110(7):921-937.
  4. Mohamed AA, Shousha WG, Shaker O, et al. Role of Serum Adiponectin, IL-6 and hsCRP in Nonalcoholic Fatty Liver Egyptian Patients. International Journal of Biochemistry Research & Review. 2014;4(6):493-504
  5. Tana C, Ballestri S, Ricci F, et al. Cardiovascular Risk in Non-Alcoholic Fatty Liver Disease: Mechanisms and Therapeutic Implications. Int J Environ Res Public Health. 2009;16:3104
  6. Francque SM, Graaff DVD, Kwanten WJ. Non-alcoholic Fatty Liver Disease and Cardiovascular risk: Pathophysiological Mechanism and Implications. J Hepatol. 2016; 65(2): 425-43.
  7. Wong CR, Lim JK. The Association Between Nonalcoholic Fatty Liver Disease and Cardiovascular Disease Outcomes. Clinical Liver Disease. 2018;12(2): 39-44.
  8. Adams LA, Anstee QM, Tilg H, et al. Non-alcoholic Fatty Liver Disease and its Relationship with Cardiovascular Disease and Other Extrahepatic Diseases. Gut. 2017;66:1138-1153
  9. Targher G, Bertolini L, Padovani R, et al. Relations between carotid artery wall thickness and liver histology in subjects with nonalcoholic fatty liver disease. Diabetes Care. 2006;29:1325-1330
  10. Chen Z, Yu Y, Cai J, et al. Emerging molecular targets for treatment of nonalcoholic fatty liver disease. Trends Endocrinol Metab. 2019;30:903-914
  11. Cavalca V, Cighetti G, Bamonti F, et al. Oxidative Stress and Homocysteine in Coronary Artery Disease. Clin Chem. 2001;47:887-892
  12. Ahmed MH, Barakat S, Almobarak AO. Nonalcoholic Fatty Liver Disease and Cardiovascular Disease: Has the Time Come for Cardiologists to Be Hepatologists. Journal of Obesity. 2012
  13. Oni ET, Agatston AS, Blaha MJ, et al. A systematic review: burden and severity of subclinical cardiovascular disease among those with nonalcoholic fatty liver; should we care? Atherosclerosis. 2013;230:258-267
  14. Sookoian S, Pirola CJ. Nonalcoholic fatty liver disease is strongly associated with carotid atherosclerosis: a systematic review. J Hepatol. 2008;49:600-607
  15. Mishra S, Yadav D, Gupta M, et al. A study of carotid atherosclerosis in patients with non-alcoholic fatty liver disease. Indian J Clin Biochem. 2013;28:79-83
  16. Jannah BM, Isdadiyanto S, Sitasiwi AJ. Histopathological of White Rats Aorta Induced by High-Fat-Feed After Administered by Neem Leaf Ethanolic Extract. Biosaintifika: Journal of Biology Education. 2020;12(3):370-378
  17. Aisyah S, Balqis U, Friyan EK. Histopatologi Jantung Tikus Putih (Rattus norvegicus) Akibat Pemberian Minyak Jelantah. Jurnal Medika Veterinaria. 2014;8(1):87-90.

How to Cite

Ahmadwirawan, M. P., Cangara, H., Santoso, A., Hardjo, M., Adnan, E., & Hamid, F. (2021). Analysis of Interleukin-6 level in Serum and Histopathology Changes of Cardiovascular Tissue on Male Wistar Rat with Nonalcoholic Fatty Liver Disease (NAFLD). Indonesia Journal of Biomedical Science, 15(2), 174–179. https://doi.org/10.15562/ijbs.v15i2.321
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