Original Articles

Analysis of Interleukin-6 level in Serum and Histopathology Changes of Cardiovascular Tissue on Male Wistar Rat with Nonalcoholic Fatty Liver Disease (NAFLD)

Mylda Pratiwi Ahmadwirawan , Husni Cangara, Arif Santoso, Marhaen Hardjo, Endy Adnan, Firdaus Hamid

Mylda Pratiwi Ahmadwirawan
Aging and Regenerative Medicine Concentration, Biomedical Magister Program, Universitas Hasanuddin, Makassar, Indonesia. Email: myldapratiwi@gmail.com

Husni Cangara
Department of Pathology Anatomy, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia

Arif Santoso
Department of Pulmonology and Respiratory Medicine, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia

Marhaen Hardjo
Department of Biochemistry, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia

Endy Adnan
Department of Internal Medicine, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia

Firdaus Hamid
Department of Microbiology, Faculty of Medicine, Universitas Hasanuddin, Makassar, Indonesia
Online First: December 22, 2021 | Cite this Article
Ahmadwirawan, M., Cangara, H., Santoso, A., Hardjo, M., Adnan, E., Hamid, F. 2021. Analysis of Interleukin-6 level in Serum and Histopathology Changes of Cardiovascular Tissue on Male Wistar Rat with Nonalcoholic Fatty Liver Disease (NAFLD). Indonesia Journal of Biomedical Science 15(2): 174-179. DOI:10.15562/ijbs.v15i2.321


Background: Nonalcoholic fatty liver disease (NAFLD) is the most common chronic hepatopathy and a global health issue. Interleukin-6 (IL-6) in liver pathology is very complex, and its participation in the development of NAFLD remains unclear. This study aimed to analyze the relationship between NAFLD and IL-6 as one of inflammaging biomarkers along with CVD histopathology.

Methods: This study used a post-test control group design. The subject of this study was 14 male Wistar rats and divided into two groups. The control group was given standard diet and the NAFLD-modelling group was given 8 weeks high-fat-high-fructose diet. Both groups will be terminated at the 8th week for organ histopathological examination.

Results: The IL-6 levels in NAFLD rats (16.14±6.46ng/ml) and non-NAFLD rats (11.28±3.49ng/ml). The blood vessel wall thickness of NAFLD (1.47±0.33 µm) and non-NAFLD rats (1.70±0.28 µm). The diameter of the blood vessels of NAFLD rats (14.06±3.30 m) and non-NAFLD rats (15.66±1.02 m). It was found that there were differences in cardiac histopathology (hemorrhagic (p:0.003), degenerated (p:0.036), and hyperemic(p:0.003)) between NAFLD and non-NAFLD modeling rats. As for IL-6 levels, blood vessel wall thickness, diameter, and heart histopathology (necrosis) there was no difference between NAFLD and non-NAFLD modeling rats (p>0.05).

Conclusion: It has shown that NAFLD rats significantly caused cardiac histopathological changes. The levels of IL-6 tend to be higher in NAFLD rats but not significant, as well as the diameter of coronary artery shows a small tendency in NAFLD rats.

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