Original Articles

The effectiveness of Triamcinolone Acetonide (TA) and Interleukin-6 (IL-6) levels in preventing intraperitoneal adhesion

Yan Senjaya , Reno Rudiman, Bambang Am Am Setya Sulthana

Yan Senjaya
Department of Surgery, Dr. Hasan Sadikin General Hospital, Universitas Padjadjaran, Bandung, West Java, Indonesia. Email: fx_yan_senjaya@yahoo.com

Reno Rudiman
Department of Surgery, Dr. Hasan Sadikin General Hospital, Universitas Padjadjaran, Bandung, West Java, Indonesia

Bambang Am Am Setya Sulthana
Department of Surgery, Dr. Hasan Sadikin General Hospital, Universitas Padjadjaran, Bandung, West Java, Indonesia
Online First: August 24, 2021 | Cite this Article
Senjaya, Y., Rudiman, R., Sulthana, B. 2021. The effectiveness of Triamcinolone Acetonide (TA) and Interleukin-6 (IL-6) levels in preventing intraperitoneal adhesion. Indonesia Journal of Biomedical Science 15(2): 109-112. DOI:10.15562/ijbs.v15i2.329

Background: Intraperitoneal adhesions are formed due to the inflammatory response in releasing proinflammatory cytokines such as IL-6. Triamcinolone Acetonide (TA) acts as an anti-inflammatory by inhibiting the production and release of IL-6 cytokines. This study aims to determine the effectiveness of triamcinolone acetonide in preventing intraperitoneal adhesions and its effect on assessing IL-6 levels in post-exploratory laparotomy in rats.

Methods: An experimental study was conducted among 40 male Wistar rats from January and March 2021 in the Animal Laboratory of Universitas Padjadjaran, Bandung, Indonesia. Blood samples were taken before treatment and on the 14th day after treatment. In rats, laparotomy and cecum abrasion were performed with a needle. Rats in the treatment group were given triamcinolone acetonide at a dose of 0.1 mg, 0.3 mg, or 0.5 mg. On the 14th day, a microscopic examination of the degree of adhesion was performed. Data were analyzed using SPSS version 20 for Windows.

Results: The results of the Kruskal-Wallis test analysis on the formation of intraperitoneal adhesions microscopically showed a significant difference between the control group and the treatment group (p < 0.001) with the lowest mean rank in the TA group of 0.3 mg (11.4), which stated that this dose was more effective in preventing intraperitoneal adhesions. The One-Way ANOVA test on IL-6 examination had a significant difference in postoperative IL-6 reduction between the control and treatment groups (p < 0.001).

Conclusion: Triamcinolone acetonide was effective in preventing intraperitoneal adhesions and reducing IL-6 levels in post-exploratory laparotomy rats.


van Steensel S, van den Hil LCL, Schreinemacher MHF, Ten Broek RPG, van Goor H, Bouvy ND. Adhesion awareness in 2016: An update of the national survey of surgeons. PLoS One. 2018;13(8):e0202418.

Tabibian N, Swehli E, Boyd A, Umbreen A, Tabibian JH. Abdominal adhesions: A practical review of an often overlooked entity. Ann Med Surg (Lond). 2017;15:9-13.

Sungkar A, Widyatmoko D, Yarso KY, Wasita B. The effect of duration of wound skin tissue on MDA, TNF-?, IL-6, Caspase 3, VEGF levels, and granulation tissue thickness in the white rat (Rattus novergicus). Bali Medical Journal. 2020;9(3):918-923.

van Steensel S, Liu H, Mommers EHH, Lenaerts K, Bouvy ND. Comparing Five New Polymer Barriers for the Prevention of Intra-abdominal Adhesions in a Rat Model. J Surg Res. 2019;243:453-459.

Brochhausen C, Schmitt VH, Planck CN, Rajab TK, Hollemann D, Tapprich C, et al. Current strategies and future perspectives for intraperitoneal adhesion prevention. J Gastrointest Surg. 2012;16(6):1256-74.

Atta HM. Prevention of peritoneal adhesions: a promising role for gene therapy. World J Gastroenterol. 2011;17(46):5049-5058.

Roberts LM, Sanfilippo JS, Raab S. Effects of laparoscopic lavage on adhesion formation and peritoneum in an animal model of pelvic inflammatory disease. J Am Assoc Gynecol Laparosc. 2002;9(4):503-507.

Saharui A, Lahunduitan I, Kalitouw F. Peranan triamnicolone acetate terhadap adhesi intraperitoneal pasca laparotomi. Jurnal Biomedik: JBM. 2017;9(1):13-6.

Arung W, Meurisse M, Detry O. Pathophysiology and prevention of postoperative peritoneal adhesions. World J Gastroenterol. 2011;17(41):4545-4553.

Kim SG, Song KY, Lee HH, Kim EY, Lee JH, Jeon HM, et al. Efficacy of an antiadhesive agent for the prevention of intra-abdominal adhesions after radical gastrectomy: A prospective randomized, multicenter trial. Medicine (Baltimore). 2019;98(19):e15141.

Takagi K, Araki M, Fukuoka H, Takeshita H, Hidaka S, Nanashima A, et al. Novel powdered anti-adhesion material: preventing postoperative intra-abdominal adhesions in a rat model. Int J Med Sci. 2013;10(4):467-74.

Siebelt M, Korthagen N, Wei W, Groen H, Bastiaansen-Jenniskens Y, Müller C, et al. Triamcinolone acetonide activates an anti-inflammatory and folate receptor-positive macrophage that prevents osteophytosis in vivo. Arthritis Res Ther. 2015;17:352.

Iwasaki K, Ahmadi AR, Qi L, Chen M, Wang W, Katsumata K, Tsuchida A, Burdick J, Cameron AM, Sun Z. Pharmacological Mobilization and Recruitment of Stem Cells in Rats Stops Abdominal Adhesions After Laparotomy. Sci Rep. 2019;9(1):7149

Beyene RT, Kavalukas SL, Barbul A. Intra-abdominal adhesions: Anatomy, physiology, pathophysiology, and treatment. Curr Probl Surg. 2015;52(7):271-319.

Fielding CA, Jones GW, McLoughlin RM, McLeod L, Hammond VJ, Uceda J, et al. Interleukin-6 signaling drives fibrosis in unresolved inflammation. Immunity. 2014;40(1):40-50.

Grellner W, Georg T, Wilske J. Quantitative analysis of proinflammatory cytokines (IL-1beta, IL-6, TNF-alpha) in human skin wounds. Forensic Sci Int. 2000;113(1-3):251-264.

Saba AA, Kaidi AA, Godziachvili V, Dombi GW, Dawe EJ, Libcke JH, et al. Effects of interleukin-6 and its neutralizing antibodies on peritoneal adhesion formation and wound healing. Am Surg. 1996;62(7):569-572.

No Supplementary Material available for this article.
Article Views      : 0
PDF Downloads : 0