Identification of active chemical compounds and potential antibacterial snail mucus (Achatina fulica) on bacteria Enterococcus foecalis causes of periodontitis

I Gusti Agung Ayu Putu Swastini; Ni Nengah  Sumerti; Ni Ketut  Ramini; Wayan  Juniarsana; I Ketut  Suardana; Ida Ayu Made Sri  Arjani; Ida Ayu Dewi Kumala  Ratih; Regina  Tedjasulaksana

doi:10.15562/ijbs.v16i2.426

Identification of active chemical compounds and potential antibacterial snail mucus (Achatina fulica) on bacteria Enterococcus foecalis causes of periodontitis

I Gusti Agung Ayu Putu Swastini ,

Ni Nengah Sumerti ,

Ni Ketut Ramini ,

Wayan Juniarsana ,

I Ketut Suardana ,

Ida Ayu Made Sri Arjani ,

Ida Ayu Dewi Kumala Ratih ,

Regina Tedjasulaksana ,

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DOI: https://doi.org/10.15562/ijbs.v16i2.426 |
Published: 2022-12-10

Abstract

Background: A chemical compound is a chemical compound present in a natural source that gives it the special properties and characteristics of the plant or animal source. Snail slime is one of the traditional medicines from animals that is used by the community as a traditional medicine for healing minor wounds and toothaches. The aim of this study was to identify the active chemical compounds and antibacterial potential of snail mucus against the bacteria E. foecalis that causes periodontitis.

Method: True experiment laboratory research method which is carried out by laboratory analysis of snail mucus to obtain chemical elements, the content of compounds contained in a test sample. The content of active compounds based on the Gas Chromatography-Mass Spectrometry (GCMS) test.

Results: The average content of heparan sulfate with four repetitions was 16.45 mg/100g, Acharan sulfate 21.33 mg/100g, protein achasin 102.22 mg/100g, Glycoconyugat 88.6 mg/100g, Ion Ca2+ 86.2 mg/100g, Beta agglutinins 85.22 mg/100g. Toxicity test was carried out with four repetitions with concentrations of 100%, 50%, 25%, and 12.5%. And the lowest results of the toxicity test were at a concentration of 100% with a value of 0.171, while at a concentration of 50% 0.302, 25% 0.453, and 12.5% 0.768, for bacteria E. Foecalis with an inhibitory power of 23.15 mm, the category was very strong.

Conclusion: The active chemical content in snail mucus is Acharan sulfate, achasin protein, Glycoconyugat, Ca2+ ion, Beta agglutinin, and the antibacterial potential of snail mucus against E. foecalis bacteria is very strong.

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