Skip to main content Skip to main navigation menu Skip to site footer

Identification of active chemical compounds and potential antibacterial snail mucus (Achatina fulica) on bacteria Enterococcus foecalis causes of periodontitis

Abstract

Background: A chemical compound is a chemical compound present in a natural source that gives it the special properties and characteristics of the plant or animal source. Snail slime is one of the traditional medicines from animals that is used by the community as a traditional medicine for healing minor wounds and toothaches. The aim of this study was to identify the active chemical compounds and antibacterial potential of snail mucus against the bacteria E. foecalis that causes periodontitis.

Method: True experiment laboratory research method which is carried out by laboratory analysis of snail mucus to obtain chemical elements, the content of compounds contained in a test sample. The content of active compounds based on the Gas Chromatography-Mass Spectrometry (GCMS) test.

Results: The average content of heparan sulfate with four repetitions was 16.45 mg/100g, Acharan sulfate 21.33 mg/100g, protein achasin 102.22 mg/100g, Glycoconyugat 88.6 mg/100g, Ion Ca2+ 86.2 mg/100g, Beta agglutinins 85.22 mg/100g. Toxicity test was carried out with four repetitions with concentrations of 100%, 50%, 25%, and 12.5%. And the lowest results of the toxicity test were at a concentration of 100% with a value of 0.171, while at a concentration of 50% 0.302, 25% 0.453, and 12.5% 0.768, for bacteria E. Foecalis with an inhibitory power of 23.15 mm, the category was very strong.

Conclusion: The active chemical content in snail mucus is Acharan sulfate, achasin protein, Glycoconyugat, Ca2+ ion, Beta agglutinin, and the antibacterial potential of snail mucus against E. foecalis bacteria is very strong.

Section

References

  1. Soraya C, Sunnati, Wulandari F. Antibacterial Effects of Neem Leaf Extract (Azadirachta indica) Against Enterococcus faecalis Growth In Vitro. Cakradonya Dent J. 2019;11(1):23-32.
  2. Berniyanti T, Suwarno. Characterization of Local Isolate Snail Mucus Protein (Achasin) as Antibacterial Factor. Media Kedokt Hewan. 2007;23(3):139-144.
  3. Bagaskara DH. Use of Snail Mucus (Achatina fulica) in Accelerating the Wound Healing Process, Research Article, Faculty of Psychology. Uiversitas Negri Semarang. Semarang; 2009.
  4. Manson JD, Eley BM. Textbook of Periodonti. Jakarta: Hipokrates; 2013.
  5. Ernawati GC, Tantin G. Potential of Robusta Coffee Bean Extract (Coffea Robusta) Gel on TNF A Reaction in Wistar Mice Periodntits Induced by Phorpyromonas gingivalis. Unversitas Jember; 2013.
  6. Suarsana N, Winaya W, Suardana S, Priyono A. Histochemical Study of Changes in Glycoconjugate Distribution Patterns in Epithelial and Glandular Cells of the Chicken Intestine in Stress and Disease Conditions. Competency Grant Research Report A2. Universitas Udayana; 2005.
  7. Harti AS, Murharyati A, Dwi Sulisetyawati S, Oktariani M. The effectiveness of snail mucus (Achatina fulica) and chitosan toward lymphocyte proliferation in vitro. Asian J Pharm Clin Res. 2018;11(Special Issue 313 3):85-88.
  8. Vieira TCRG, Costa-Filho A, Salgado NC. Acharan sulfate, the new glycosaminoglycan from Achatina fulica Bowdich 1822: Structural heterogeneity, metabolic labeling and localization in the body, mucus and the organic shell matrix. Eur J Biochem. 2004;271(4):845-854.
  9. Denny N, Mieke HS. The role of Enterococcus faecalis in the persistence of root canal infection. Universitas Padjajaran Bandung; 2013.
  10. Davis WW, Stout TR. Disc Plate Method of Microbiological Antibiotic Assay. Applied Microbiology. 1971;22(1):659-665.

How to Cite

Swastini, I. G. A. A. P., Sumerti, N. N. ., Ramini, N. K. ., Juniarsana, W. ., Suardana, I. K. ., Arjani, I. A. M. S. ., Ratih, I. A. D. K. ., & Tedjasulaksana, R. . (2022). Identification of active chemical compounds and potential antibacterial snail mucus (Achatina fulica) on bacteria Enterococcus foecalis causes of periodontitis. Indonesia Journal of Biomedical Science, 16(2), 102–105. https://doi.org/10.15562/ijbs.v16i2.426

HTML
63

Total
75

Share

Search Panel

I Gusti Agung Ayu Putu Swastini
Google Scholar
Pubmed
IJBS Journal


Ni Nengah Sumerti
Google Scholar
Pubmed
IJBS Journal


Ni Ketut Ramini
Google Scholar
Pubmed
IJBS Journal


Wayan Juniarsana
Google Scholar
Pubmed
IJBS Journal


I Ketut Suardana
Google Scholar
Pubmed
IJBS Journal


Ida Ayu Made Sri Arjani
Google Scholar
Pubmed
IJBS Journal


Ida Ayu Dewi Kumala Ratih
Google Scholar
Pubmed
IJBS Journal


Regina Tedjasulaksana
Google Scholar
Pubmed
IJBS Journal