Skip to main content Skip to main navigation menu Skip to site footer

Association of urine Interleukin-8 (IL-8) with renal impairment in lupus patients

  • Emanuel Hananto ,
  • Laura Christiani ,
  • Dominica Pita Sari ,
  • Umi Kalsum ,
  • Kusworini ,
  • Hani Susianti ,

Abstract

Background: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with clinical manifestations that vary between individuals with progressive and irreversible SLE organ damage. Recently, it has become known that Interleukin-8 (IL-8) levels in the blood rise in SLE patients compared to healthy people. The increase of IL-8 causes kidney inflammation by promoting the release of the Neutrophil Extracellular Traps (NET) complex, which supports further renal damage. This study aims to evaluate the association of urine interleukin-8 with renal impairment in SLE patients.

Methods: A total of 45 lupus participants—22 with and 23 without renal abnormalities—had their urine collected. The interleukin-8 concentration was measured using an enzyme-linked immunosorbent assay (ELISA)—renal impairment criteria based on renal biopsy or ACR 1997 criteria. Mann-Whitney and Spearman correlation tests were employed in the statistical analysis. Data were analyzed using SPSS version 25.0 for Windows.

Results: There was no significant difference in IL-8 urine levels between the SLE group with renal impairment  (111.27±59.03 pg/ml) and the SLE group without renal impairment (125.76±66.62 pg/ml) was not significant (p=0.67). Urine IL-8 also did not significantly correlate with blood neutrophil count, leukocyte count and leukocyturia (p>0.05).

Conclusion: In lupus patients, urine Interleukin-8 levels are not associated with renal impairment.

Section

References

  1. Cimbaluk D, Naumann A. Renal involvement in systemic lupus erythematosus: glomerular pathology, classification, and future directions. Diagnostic Histopathology. 2017;23(3):109-16.
  2. Mok CC. Biomarkers for lupus nephritis: a critical appraisal. Journal of Biomedicine and Biotechnology. 2010;2010:638413.
  3. Davidson A. What is damaging the kidney in lupus nephritis?. Nature Reviews Rheumatology. 2016;12(3):143-153.
  4. David JM, Dominguez C, Hamilton DH, Palena C. The IL-8/IL-8R axis: a double agent in tumor immune resistance. Vaccines. 2016;4(3):22.
  5. Mao YM, Zhao CN, Liu LN, Wu Q, Dan YL, Wang DG, et al. Increased circulating interleukin-8 levels in systemic lupus erythematosus patients: a meta-analysis. Biomarkers in Medicine. 2018;12(11):1291-1302.
  6. Salazar-Gonzalez H, Zepeda-Hernandez A, Melo Z, Saavedra-Mayorga DE, Echavarria R. Neutrophil extracellular traps in the establishment and progression of renal diseases. Medicina. 2019;55(8):431.
  7. Almaani S, Meara A, Rovin BH. Update on lupus nephritis. Clinical Journal of the American Society of Nephrology. 2017;12(5):825-835.
  8. Aragón CC, Tafúr RA, Suárez-Avellaneda A, Martínez MT, de Las Salas A, Tobón GJ. Urinary biomarkers in lupus nephritis. Journal of Translational Autoimmunity. 2020;3:100042.
  9. Aringer M, Petri M. New classification criteria for systemic lupus erythematosus. Current Opinion in Rheumatology. 2020;32(6):590-596.
  10. Fanouriakis A, Tziolos N, Bertsias G, Boumpas DT. Update οn the diagnosis and management of systemic lupus erythematosus. Annals of The Rheumatic Diseases. 2021;80(1):14-25.
  11. Shepherd E. Specimen collection 1: general principles and procedure for obtaining a midstream urine specimen. Nursing Times. 2017;113(7):45-47.
  12. Remick DG. Interleukin-8. Critical care medicine. 2005;33(12):S466-S467.
  13. Saba R, Sorensen DL, Booth SA. MicroRNA-146a: A Dominant, Negative Regulator of the Innate Immune Response. Front Immunol. 2014;5:578.
  14. Bhaumik D, Scott GK, Schokrpur S, Patil CK, Orjalo AV, Rodier F, et al. MicroRNAs miR-146a/b negatively modulate the senescence-associated inflammatory mediators IL-6 and IL-8. Aging. 2009;1(4):402-411.
  15. Lan CC, Wu CS, Huang SM, Wu IH, Chen GS. High-glucose environment enhanced oxidative stress and increased interleukin-8 secretion from keratinocytes: new insights into impaired diabetic wound healing. Diabetes. 2013;62(7):2530-2538.
  16. Yuliyani EA, Sutanegara SWD, Muliartha IM. Effectiveness of nasal irrigation with isotonic saline against interleukin-8 levels and quality of life in chronic rhinosinusitis patients. Bali Medical Journal. 2019;8(3):750-754.
  17. Sudrajad H, Mudigdo A, Purwanto B, Setiamika M. Ethanol extract of propolis decreases the Interleukin-8 (IL-8) expression and blood Malondialdehyde (MDA) level in otitis media rat model induced by Pseudomonas aeruginosa. Bali Medical Journal. 2020;9(2):504-510.
  18. Subawa AAN, Samatra DPGP, Santhi DGDDS. Relationship between Interleukin-18 (IL-18) level and Mean Platelet Volume (MPV) with ischemic stroke event in Sanglah General Hospital, Bali, Indonesia. Bali Medical Journal. 2020;9(2):477-481.

How to Cite

Hananto, E. ., Christiani, L. ., Sari, D. P. ., Kalsum, U. ., Kusworini, & Susianti, H. . (2023). Association of urine Interleukin-8 (IL-8) with renal impairment in lupus patients. Indonesia Journal of Biomedical Science, 17(2), 202–205. https://doi.org/10.15562/ijbs.v17i2.430

HTML
66

Total
94

Share