Nano extract of Coriandrum sativum l (ketumbar seed) decrease malondialdehyde (MDA) and blood glucose, and increase superoxide dismutase (SOD) in hyperglycaemic wistar rats

Sri Wahjuni; Ida Ayu Raka Astitiasih; Ni Made Puspawati; Mustika Lahaya; A.A. Bawa Putra; Ida Bagus Putra Manuaba; Ahmad Fudholi

doi:10.15562/ijbs.v16i2.442

Nano extract of Coriandrum sativum l (ketumbar seed) decrease malondialdehyde (MDA) and blood glucose, and increase superoxide dismutase (SOD) in hyperglycaemic wistar rats

Sri Wahjuni ,

Ida Ayu Raka Astitiasih ,

Ni Made Puspawati ,

Mustika Lahaya ,

A.A. Bawa Putra ,

Ida Bagus Putra Manuaba ,

Ahmad Fudholi ,

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DOI: https://doi.org/10.15562/ijbs.v16i2.442 |
Published: 2022-12-30

Abstract

Introduction: Coriandrum sativum L (ketumbar seeds) nano extract was prepared using chitosan as a nano extract framework and tripolyphosphate as a stabilizer by ionic gelatin method. This study aimed to reduce free radicals in streptozotocin-induced hyperglycaemic rats by administering Coriandrum sativum L nano extract as nano herbal.

Methods: Characterization of the resulting nano extract was performed using Fourier Transformation Infra-Red (FTIR). The ability of nano extract as antihyperglycemic on streptozotocin-induced rats was tested using the randomized post-test only control group design research by observing the levels of blood glucose, MDA, and SOD as markers of oxidative stress.

Results: The phytochemical test showed that Coriandrum sativum L extracts contained polyphenols, flavonoids, alkaloids, and terpenoids. Characterization with PSA strongly supports nano extract as a delivery system for bioactive substances with a zeta potential of -25.80 mV and a particle size of 455.20 nm. The result of the antihyperglycemic test showed that oral administration at a dose of 50 mg/Kg BW/day gave the best result for decreasing glucose blood and MDA, and increasing SOD level in hyperglycaemic rats. LCMS/MS analysis showed that in ethanol extract of Coriandrum sativum L are suspected to contain the 7 compounds: DL-Valine, indoline, 2-quinolinol, trans-tnrthole, luteolin, apigenin, linoliamide, and luteolinin are compounds known in to be able to lower blood glucose and malondialdehyde.

Conclusion: Coriandrum sativum L (ketumbar seeds) nano extract was prepared using chitosan had potential to decrease MDA and blood glucose, and increase SOD level in hyperglycaemic rats.

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