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Identification and quantification of Roseburia intestinalis and Escherichia coli in hypertensive patients in Denpasar



Abstract

Background: Low-grade chronic inflammation may contribute to the development of atherosclerosis as well as hypertension by inducing the production of pro-inflammatory cytokines. Several studies have revealed a relationship between the microbiota living in the gut and the incidence of hypertension. Two of several gut microbiota that contributed, in this case, are Escherichia coli (E. coli) and Roseburia intestinalis (R. intestinalis). Therefore this study aimed to identify and quantify gut microbes namely R. intestinalis and E.coli in hypertensive patients in Bali, especially in Denpasar City.

Methods: This research is a cross-sectional study located at South Denpasar Health Center I, Bali, and the Integrated Biomedical Laboratory, Faculty of Medicine, Universitas Udayana. The sample in this study were hypertensive patients who had been registered at South Denpasar Health Center I and who met the inclusion criteria and did not meet the exclusion criteria. The E. coli and R. intestinalis concentrations were assessed by conducting DNA isolation and RT-qPCR. Statistical analysis using univariate analysis, bivariate analysis using the Independent T-Test; Mann-Whitney Test; Chi-Square, and ROC analysis

Results: There were 70 subjects included in this study. Thirty-one subjects were hypertensive patients and the other 39 patients were not hypertensive. The age of the subjects between the two groups was found statistically significant (p=0.007). We also found that comorbid were found mostly in hypertensive patients (p=0.009). Most of the comorbid experienced among them were cardiovascular diseases and diabetes mellitus. Hypertensive subjects had more E. coli concentration in their gut compared with non-hypertensive subjects (p=0.048), while R. intestinalis concentrations were similar in both groups (p>0.05). From the multivariate analysis, it was found that hypertensive patients tended to have a higher concentration of E. coli than non-hypertensive patients (p=0.012; 95% CI:0.055–0.417).

Conclusion: E. coli concentration in the gut is independently associated with the incidence of hypertension, while R. intestinalis concentration is not.

Keywords: Roseburia intestinalis Escherichia coli hypertension gut microbiota
Section

References

  1. WHO. Noncommunicable diseases country profiles 2018. Geneva PP - Geneva: World Health Organization; 2018.
  2. Mills KT, Stefanescu A, He J. The global epidemiology of hypertension. Nat Rev Nephrol. 2020;16(4):223–37.
  3. Verhaar BJH, Prodan A, Nieuwdorp M, Muller M. Gut microbiota in hypertension and atherosclerosis: A review. Nutrients. 2020;12(10):1–22.
  4. Helmyati S, Wisnusanti SU, Wigati M, Yuliati E. the Relation Between Gut Microbiota and Obesity Among Children in West Lombok, West Nusa Tenggara, Indonesia. Media Gizi Mikro Indonesia. 2019;11(1):12.
  5. Afriansyah MA, Budayanti NNS, Pinatih KJP. Detection of metallo-Î2-lactamase gene blaIMP of Escherichia coli clinical isolates in Sanglah General Hospital Bali. Indonesia Journal of Biomedical Science. 2019;13(2).
  6. Huart J, Leenders J, Taminiau B, Descy J, Saint-Remy A, Daube G, et al. Gut Microbiota and Fecal Levels of Short-Chain Fatty Acids Differ Upon 24-Hour Blood Pressure Levels in Men. Hypertension. 2019;74(4):1005–13.
  7. De la Cuesta-Zuluaga J, Mueller NT, Álvarez-Quintero R, Velásquez-Mejía EP, Sierra JA, Corrales-Agudelo V, et al. Higher Fecal Short-Chain Fatty Acid Levels Are Associated with Gut Microbiome Dysbiosis, Obesity, Hypertension and Cardiometabolic Disease Risk Factors. Vol. 11, Nutrients. 2019.
  8. Yan Q, Gu Y, Li X, Yang W, Jia L, Chen C, et al. Alterations of the gut microbiome in hypertension. Front Cell Infect Microbiol. 2017;7(AUG):1–9.
  9. Lim MY, Park YS, Kim JH, Nam Y Do. Evaluation of fecal DNA extraction protocols for human gut microbiome studies. BMC Microbiol. 2020;20(1).
  10. Kurakawa T, Ogata K, Matsuda K, Tsuji H, Kubota H, Takada T, et al. Diversity of intestinal Clostridium coccoides group in the Japanese population, as demonstrated by reverse transcription-quantitative PCR. PLoS One. 2015;10(5).
  11. Morin NJ, Gong Z, Li XF. Reverse transcription-multiplex PCR assay for simultaneous detection of Escherichia coli O157:H7, Vibrio cholerae O1, and Salmonella Typhi. Clin Chem. 2004;50(11):2037–44.
  12. Dan X, Mushi Z, Baili W, Han L, Enqi W, Huanhu Z, et al. Differential Analysis of Hypertension-Associated Intestinal Microbiota. Int J Med Sci. 2019;16(6):872–81.
  13. de la Cuesta-Zuluaga J, Mueller NT, Álvarez-Quintero R, Velásquez-Mejía EP, Sierra JA, Corrales-Agudelo V, et al. Higher fecal short-chain fatty acid levels are associated with gut microbiome dysbiosis, obesity, hypertension and cardiometabolic disease risk factors. Nutrients. 2019;11(1).
  14. Li J, Zhao F, Wang Y, Chen J, Tao J, Tian G, et al. Gut microbiota dysbiosis contributes to the development of hypertension. Microbiome. 2017;5(1):14.
  15. Sun S, Lulla A, Sioda M, Winglee K, Wu MC, Jacobs DRJ, et al. Gut Microbiota Composition and Blood Pressure. Hypertension. 2019;73(5):998–1006.
  16. Verhaar BJH, Collard D, Prodan A, Levels JHM, Zwinderman AH, Bäckhed F, et al. Associations between gut microbiota, faecal short-chain fatty acids, and blood pressure across ethnic groups: the HELIUS study. Eur Heart J. 2020;41(44):4259–67.
  17. Yan Q, Gu Y, Li X, Yang W, Jia L, Chen C, et al. Alterations of the Gut Microbiome in Hypertension. Front Cell Infect Microbiol. 2017;7:381.
  18. Chaudhary S, Khurana S, Mane B. Escherichia coli: Animal Foods and Public Health-Review. Journal of Microbiology, Immunology and Biotechnology. 2014;1(December 2014):31–46.
  19. Verhaar BJH, Prodan A, Nieuwdorp M, Muller M. Gut microbiota in hypertension and atherosclerosis: A review. Nutrients. 2020;12(10):1–22.
  20. Masson GS, Nair AR, Dange RB, Silva-Soares PP, Michelini LC, Francis J. Toll-like receptor 4 promotes autonomic dysfunction, inflammation and microglia activation in the hypothalamic paraventricular nucleus: Role of endoplasmic reticulum stress. PLoS One. 2015;10(3):1–15.
  21. Shen Z, Zhu C, Quan Y, Yang J, Yuan W, Yang Z, et al. Insights into Roseburia intestinalis which alleviates experimental colitis pathology by inducing anti-inflammatory responses. Journal of Gastroenterology and Hepatology (Australia). 2018;33(10):1751–60.
  22. Tamanai-Shacoori Z, Smida I, Bousarghin L, Loreal O, Meuric V, Fong SB, et al. Roseburia spp.: A marker of health? Future Microbiol. 2017;12(2):157–70.
  23. Hold GL, Schwiertz A, Aminov RI, Blaut M, Flint HJ. Oligonucleotide probes that detect quantitatively significant groups of butyrate-producing bacteria in human feces. Appl Environ Microbiol. 2003;69(7):4320–4.
  24. Duncan SH, Holtrop G, Lobley GE, Calder AG, Stewart CS, Flint HJ. Contribution of acetate to butyrate formation by human faecal bacteria. Br J Nutr. 2004;91(6):915–23.
  25. Huart J, Cirillo A, Saint-Remy A, Krzesinski J-M, de Tullio P, Jouret F. The faecal abundance of short chain fatty acids is increased in men with a non-dipping blood pressure profile. Acta Cardiol. 2021;1–4.
  26. Tsimihodimos V, Gonzalez-Villalpando C, Meigs JB, Ferrannini E. Hypertension and Diabetes Mellitus. Hypertension. 2018;71(3):422–8.
  27. Ferrannini E, Cushman WC. Diabetes and hypertension: the bad companions. The Lancet. 2012;380(9841):601–10.
  28. Buford TW. Hypertension and aging. Ageing Res Rev. 2016;26:96–111.
  29. Wadley AJ, Veldhuijzen van Zanten JJCS, Aldred S. The interactions of oxidative stress and inflammation with vascular dysfunction in ageing: the vascular health triad. Age (Dordr). 2013;35(3):705–18.
  30. Dinh QN, Drummond GR, Sobey CG, Chrissobolis S. Roles of inflammation, oxidative stress, and vascular dysfunction in hypertension. Biomed Res Int. 2014;2014:406960.
  31. Haro C, Rangel-Zúñiga OA, Alcalá-Díaz JF, Gómez-Delgado F, Pérez-Martínez P, Delgado-Lista J, et al. Intestinal Microbiota Is Influenced by Gender and Body Mass Index. PLoS One. 2016;11(5):e0154090.

How to Cite

Pinatih, K. J. P., Wihandani, D. M. ., Darwinata, A. E. ., Gede Setula Narayana, I Putu Gede Septiawan Saputra, & I Gede Putu Supadmanaba. (2023). Identification and quantification of Roseburia intestinalis and Escherichia coli in hypertensive patients in Denpasar. Indonesia Journal of Biomedical Science, 17(1), 62–67. https://doi.org/10.15562/ijbs.v17i1.457
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Komang Januartha Putra Pinatih
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Desak Made Wihandani
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Agus Eka Darwinata
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Gede Setula Narayana
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I Putu Gede Septiawan Saputra
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I Gede Putu Supadmanaba
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