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Caffeine's intracellular potential as anticancer and antiviral therapy

  • Nanda Ariane Iskandar ,
  • Febriana Catur Iswanti ,
  • Mohamad Sadikin ,


Coffee, as the most consumed drink globally, has an effect on human metabolism at extracellular and intracellular levels. Caffeine or trimethylxantine in intracellular has potential as an agent for antiviral and anticancer. Treatment with caffeine will affect the mechanism of DNA repair enzymes and induce premature chromatin condensation (PCC), which organizes the occurrence of mitosis at rudimentary cell cycle phase S which makes DNA integrity unattainable, and cells become apoptosis. Studies have already been conducted observing the effects of caffeine at the cellular level, by reacting Escherichia coli (E. coli) or mammalian cells to reactions that disrupt cell cycles such as arsenolysis, exposure to UV radiation, x-ray induced, and starvation with nothing to consumed accept of purines derivatives including caffeine. The result of caffeine treatment on E. coli and mammalian cells on UV radiation and arsenolysis least affected was inosine, acting as an inhibitor, which then can be concluded that caffeine and inosine have almost the same physiological function as substrates for substitution reactions by the most reactive cell metabolism compared to hypoxanthine, xanthine, adenine, and guanine. Additional studies explained caffeine on mammalian cells was not affecting mammalian DNA replication but only made it shorter in replication length there for giving a promised future impact for interfering mechanism of caffeine as DNA repair substrates as beneficial if it is used as a mechanism to interfere with the proliferation of cancer cells and inhibit the spread of viral virions.



  1. Ludwig IA, Clifford MN, Lean MEJ, Ashihara H, Crozier A. Coffee: Biochemistry and potential impact on health. Food Funct. 2014;5(8):1695–717.
  2. World Health Organization. 19th WHO Model List of Essential Medicines. Http://WwwWhoInt/Medicines/Publications/Essentialmedicines/En. 2015;(April):1–43.
  3. Ascherio A, Zhang SM, Hernán MA, Kawachi I, Colditz GA, Speizer FE, et al. Prospective Study of Caffeine Consumption and Risk of Parkinson’s Disease in Men and Women. Vol. 50, Ann Neurol. 2001.
  4. La Vecchia C. Coffee, liver enzymes, cirrhosis and liver cancer. Vol. 42, Journal of Hepatology. Elsevier; 2005. p. 444–6.
  5. Hubert K, Stephan M, Kerstin K. Coffee and Lower Risk of Type 2 Diabetes: Arguments for a Causal Relationship. Nutrients. 2012;13:1–17.
  6. Bønn SK, Ward NC, Hodgson JM, Croft KD. Effects of tea and coffee on cardiovascular disease risk. Vol. 3, Food and Function. 2012. p. 575–91.
  7. Cazeneuve C, Pons G, Rey E, Treluyer J, Cresteil T, Thiroux G, et al. Biotransformation of caffeine in human liver microsomes from foetuses, neonates, infants and adults. Br J Clin Pharmacol. 1994;37(5):405–12.
  8. Cornelis MC, Byrne EM, Esko T, Nalls MA, Ganna A, Paynter N, et al. Genome-wide meta-analysis identifies six novel loci associated with habitual coffee consumption. Mol Psychiatry. 2015;20(5):647–56.
  9. Arthur Koch WL. National of Oceanography. the metabolism of methylpurines by escherichia coli. 1955;
  10. Oslovsky VE, Drenichev MS, Alexeev CS, Solyev PN, Esipov RS, Mikhailov SN. Synthesis of Cytokinins via Enzymatic Arsenolysis of Purine Nucleosides. Curr Protoc Nucleic Acid Chem. 2018;75(1):1–10.
  11. Grigg S. The Reversible Suppression of Stationary Phase Mutation in E.Coli by Caffeine. 1965;(May).
  12. Fu YX, Huai H. Estimating mutation rate: How to count mutations? Genetics. 2003;164(2):797–805.
  13. Selby CP, Sancar A. Molecular mechanisms of DNA repair inhibition by caffeine. Proc Natl Acad Sci U S A. 1990;87(9):3522–5.
  14. Sancar A. Mechanisms of DNA Repair by Photolyase and Excision Nuclease (Nobel Lecture). Angewandte Chemie - International Edition. 2016;55(30):8502–27.
  15. A.R. Lehman. Effect of caffeine on DNA synthesis in Mammalian Cells. Biophys J. 1972;112(5):1977–8.
  16. Waldren CA, Patterson D. Effects of Caffeine on Purine Metabolism and Ultraviolet Light-induced Lethality in Cultured Mammalian Cells. Cancer Res. 1979;39(12):4975–82.
  17. Sliva J, Pantzartzi CN, Votava M. Inosine Pranobex: A Key Player in the Game Against a Wide Range of Viral Infections and Non-Infectious Diseases. Adv Ther. 2019;36(8):1878–905.
  18. Baynes JW, Dominiczak MH. Medical Biochemistry. Vol. 5th edition. 2018.
  19. Daniel R, Marusich E, Argyris E, Zhao RY, Skalka AM, Pomerantz RJ. Caffeine Inhibits Human Immunodeficiency Virus Type 1 Transduction of Nondividing Cells. J Virol. 2005;79(4):2058–65.
  20. Sperling K, Rao PN. The Phenomenon of Premature Chromosome Condensation: Its Relevance to Basic and Applied Research*. Vol. 23, Humangenetik. 1974.
  21. Ball LG, Xiao W. Molecular basis of ataxia telangiectasia and related diseases. Acta Pharmacol Sin. 2005;26(8):897–907.
  22. Nunnari G, Argyris E, Fang J, Mehlman KE, Pomerantz RJ, Daniel R. Inhibition of HIV-1 replication by caffeine and caffeine-related methylxanthines. Virology. 2005;335(2):177–84.
  23. Murayama M, Tsujimoto K, Uozaki M, Katsuyama Y, Yamasaki H, Utsunomiya H, et al. Effect of caffeine on the multiplication of DNA and RNA viruses. Mol Med Rep. 2008;1(2):251–5.
  24. Sukohar A, Herawati H, Sibero HT, Gigih S, Graharti R, Riyan W, et al. Effects of caffeine againts expression on Mir-423-3p in Cell Lines Hep-G2. Biomedical and Pharmacology Journal. 2018;11(1):429–35.
  25. Nishijima H, Nishitani H, Saito N, Nishimoto T. Caffeine mimics adenine and 2′-deoxyadenosine, both of which inhibit the guanine-nucleotide exchange activity of RCC1 and the kinase activity of ATR. Genes to Cells. 2003;8(5):423–35.
  26. Dasso M, Nishitani H, Kornbluth S, Nishimoto T, Newport JW. RCC1, a Regulator of Mitosis, Is Essential for DNA Replication. Vol. 12, MOLECULAR AND CELLULAR BIOLOGY. 1992.
  27. Ren X, Jiang K, Zhang F. The Multifaceted Roles of RCC1 in Tumorigenesis. Vol. 7, Frontiers in Molecular Biosciences. Frontiers Media S.A.; 2020.
  28. Ding R, Shi J, Pabon K, Scotto KW. Xanthines down-regulate the drug transporter ABCG2 and reverse multidrug resistance. Mol Pharmacol. 2012;81(3):328–37.

How to Cite

Iskandar, N. A. ., Iswanti, F. C. ., & Sadikin, M. . (2023). Caffeine’s intracellular potential as anticancer and antiviral therapy. Indonesia Journal of Biomedical Science, 17(2), 215–219.




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Nanda Ariane Iskandar
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Febriana Catur Iswanti
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Mohamad Sadikin
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